Dr Monica Schönenberger has developed a flexible, professional service unit for atomic force microscopy. As a member of the Swiss Nanoscience Institute (SNI) located at the University of Basel, she also teaches master and doctoral students to use scanning probe microscopy and gets involved in exciting new research projects. Her group performs analytical and user-related investigations on practically all kinds of surfaces. For this work, the Nano Imaging Lab (NI Lab) uses a JPK NanoWizard® 4 system.
Dr Frank Lafont heads a Research Group and is the Scientific Director of the BioImaging Center Lille (BICeL). The Group and the Center bring together the work of several institutions: Univ. of Lille, CNRS, INSERM, Hosp. Univ. Ctr. de Lille and the Institut Pasteur de Lille. Multiple imaging techniques including atomic force, confocal and multiphoton microscopy are applied to develop advanced imaging methods in the study of living cells.
Professor Guillaume Charras leads a research group at the London Centre for Nanotechnology. Their overall goal is to understand fundamental mechanical properties of cells and tissues. They are particularly interested in the interplay between signalling and cell mechanics and how mechanical changes govern cellular and embryonic morphogenesis.
Professor Luisa Mestroni heads a laboratory studying the genetics of cardiac muscle diseases. Based at the University of Colorado Denver and working in conjunction with labs in Italy, the lab’s interest is in the genes causing dilated cardiomyopathy, arrhythmogenic right ventricular cardiomyopathy/dysplasia (ARVD/C), left ventricular noncompaction and hypertrophic cardiomyopathy.
Professor Taiji Adachi heads a multidiscipline research group at Kyoto University. One of its goals is to understand the mechanical basis of mechanotransduction at multiscale. This means they carry out experimental and theoretical approaches at tissue, cell and molecular scale.
Professor Tanya Dahms leads a research group in the Faculty of Science at the University of Regina in Saskatchewan, Canada. They seek to systematically characterize the structural, chemical and physical properties of outer surfaces (i.e. cell membranes and walls) as a function of internal biochemical factors and in response to external stimuli, to help elucidate their role as a sensing organelle or “sensosome”.
Professor Itay Rousso leads a research group in the Department of Physiology and Cell Biology at Ben-Gurion University in Beer Sheba. Their focus is to uncover the physical mechanism underlying the replication machinery of enveloped retroviruses (primarily HIV).
PhD student, Christian Halbig, is a member of the research group led by Professor Dr Siegfried Eigler at the Institute of Chemistry & Biochemistry at the Free University of Berlin (FUB). The group is focused on the preparation and characterization of carbon-based nanoparticles. They mainly use single layered graphene sheets obtained from graphene oxide which are then modified by different functionalization techniques.
Professor Kyeong Kyu Kim leads a research group in the Structural Biology Laboratory of Sungkyunkwan University (SKKU) in Suwon, South Korea. The goal of their research is to understand the working mechanisms of Sox2, a master transcription factor that plays a role in controlling the “stemness” of cells.
Dr Henri Franquelim is a Post Doc researcher at the Max Planck Institute of Biochemistry situated near Munich. He is a member of Professor Petra Schwille’s research team whose goal is to quantitatively understand the essence and core principles of living systems, with the very far goal of reconstituting self-regulating biomimetic systems in vitro.
Dr Jan Pribyl is Deputy Group Leader in the research group of Professor Petr Skládal at Masaryk University, Brno in the Czech Republic. The main research aim of the group was to develop electrochemical and acoustic (QCM) biosensors.
Professor Huabing Yin is a senior lecturer in the Division of Biomedical Engineering at the University of Glasgow. Her research aims to understand how a cell interacts with its surroundings at the microscale level since this process is fundamental to a vast number of applications in health care.
Dr Gabriele Ciasca and Professor Massimiliano Papi are members of the research team of Professor Marco De Spirito in the Institute of Physics at the Catholic University of Rome, Italy. One of the main goals of their group is the investigation of how cells sense and respond to physical and mechanical stimuli.
The research of the SPM group of Prof. Jamie Hobbs is based around instrument development for scanning probe microscopy for the study of soft matter and biological systems at the molecular scale. As well as applying existing methods and instrumentation, the group have the added challenge to develop new equipment and protocols to allow the study of different samples at higher magnifications applying new detection systems.
The research team lead by Dr Mauro Modesti at the Centre for Cancer Research, Marseilles (CRCM), focuses on the study and understanding of the molecular mechanisms that assure DNA repair and the maintain of genome integrity in human cells.
Dr Kristian Franze is a lecturer in the Department of Physiology, Development and Neuroscience at the University of Cambridge. His major goal is to understand when, where and how mechanical signals, such as forces and local tissue stiffness, are involved in controlling cell development and function in the nervous system.
Dr Nuno C Santos leads the Biomembranes and Nanomedicine group at the Institute of Molecular Medicine at the University of Lisbon, Portugal. Driven by the fact that cardiovascular diseases are the leading cause of mortality worldwide, accounting for about one third of all deaths and that biomarkers for assessing cardiovascular risk still have a limited applicability, there is much potential for research into new solutions. High levels of fibrinogen, a protein essential for the blood clotting process, have been identified as a potential risk factor for these diseases and it is this topic that the group has applied atomic force microscopy (AFM) to study.
Dr Gordon McPhee is head of production for NextCell Pty in Adelaide, Australia. Situated on the Mawson Lakes Campus, he is located next to the University of South Australia (UniSA), Australia’s leading university for interdisciplinary research, where he has access to their instrumentation as part of the group of Professor Nico Voelcker at the Future Industries Institute. Here, he uses JPK’s NanoWizard® AFM system in multiple programs.
Professor Joon Won Park is head of the Supersensitive Molecular Laboratory at Korea’s Pohang University of Science & Technology. His group’s research goal is to bring AFM into the field of medical diagnostics. It is their belief that AFM is the most sensitive tool for detecting various biomarkers without amplification or labelling. Reliability and speed have been past hurdles of AFM, but Professor Park’s team has demonstrated that the application of a finely controlled surface coating to AFM probes and substrates overcomes this barrier.
Dr Marek Szymonski is a Professor at the Research Centre for Nanometer-Scale Science and Advanced Materials (NANOSAM) located at the Jagiellonian University in Krakow, Poland. The aim of his research is to understand the signals that govern intracellular communication between hepatocytes and liver sinusoidal endothelial cells (LSECs) and between cardiomyocytes and cardiac microvascular endothelial cells (CMECs).
The Group uses the JPK NanoWizard® AFM to study the interaction of bacteria with microstructured surfaces. They look at micromilled titanium surfaces. They study the adhesion of single bacteria to the surface and investigate the elastic properties of the bacteria adhered to the surface (for example, as a function of pH). The goal of all these measurements is to find the best surface structure bacteria combination to produce a biofilm reactor.
Dr Luning Liu leads a research group at the Institute of Integrative Biology at the University of Liverpool. Using interdisciplinary approaches, the Liu Lab aims to explore the molecular mechanism underlying the self-assembly, dynamics and regulation of biological membranes and macromolecular protein complexes.
Dr Yoko Yamanishi is an Associate Professor in the Department of Mechanical Engineering at Shibaura Institute of Technology, Tokyo, Japan. She leads the Yamanishi Laboratory - the Micro-nano Functional Fluid Laboratory. The Laboratory’s goals aim to clarify unknown function of cells by using micro-nano technology based on mechanical, electrical and bio-medical engineering. It targets a contribution to the development of cellular scale medical treatment.
Dr Rosa M Espinosa-Marzal is an Associate Professor in the Department of Civil & Environmental Engineering at the University of Illinois at Urbana—Champaign. The goal of her research is to design innovative systems and improved materials that can solve environmental problems of our society by applying fundamentals of surface and colloidal science, materials chemistry, and nanotechnology.
Dr Marco Fritzsche works in the Nano-Immunology group of Professor Christian Eggeling in the MRC Human Immunology Unit (HIU) at the WIMM. Their specialty areas include cell mechanics and the use of FRAP. They combine AFM with a toolbox of fluorescence microscopy techniques to investigate the mechanisms of actin cytoskeleton-mediated force generation during triggering of immune cells such as the activation of T-cells.
Established in 1976 for R&D of chemical technology in Korea, the Korean Research Institute of Chemical Technology (KRICT) has helped drive the growth of the country’s chemical industry. The focus is on the development of world-class key technologies.
Drs Christophe Demaille and Agnès Anne from CNRS work in the Laboratoire d'Electrochimie Moléculaire at the Université Paris Diderot as Group Leaders of a research team aiming to probe electron transport and communication in nanometric biostructures. Their studies are performed at the single nano-object scale using combined atomic force (AFM)-Electrochemical (SECM) microscopy.
Dr. Phillip Messersmith is the Class of 1941 Professor in the Departments of Bioengineering and Materials Science and Engineering at the University of California at Berkeley. His research group is interested in understanding structure-processing- property relationships of materials in biological systems, and in using this information to inform the design, synthesis and application of biologically inspired synthetic materials used in a variety of practical applications.
Dr Yves Dufrêne is a senior research fellow who is leading the Nanobio Team at UCL, Université catholique de Louvain. His research is at the cross-roads of nanotechnology and microbiology where the goal is to gain insight into the nanometer-scale surface architecture and molecular interactions of microbes.
Dr Meital Reches is a senior lecturer in the Institute of Chemistry. The activities in her lab focus on developing new biomolecular self-assembled systems and the study of proteins and peptides interactions with surfaces. The NanoWizard® AFM is used to perform single molecule force spectroscopy measurements.
The Schieber Research Group is led by Jay Schieber, Professor of Chemical Engineering at the Illinois Institute of Technology where he is also Director of the Center for Molecular Study of Condensed Soft Matter. Current research focuses on the kinetic theory of macromolecules to model the rheological, thermodynamic and thermal behaviour of polymeric fluids. These models are then used to predict the properties of advanced materials during and after processing.
Dr Gleb Yakubov and his team from the Rheology & Biolubrication Laboratory in the School of Chemical Engineering at the University of Queensland study properties of plant cells, e.g. how mechanical stresses influence their growth and development.
Dr Childérick Severac manages the Bionanotechnologies Platform at ITAV (Institut des Technologies Avancées en science du Vivant) on the site of the new Oncopole campus dedicated to cancer research. His research focuses on the development of soft nanolithography techniques and to develop the concept of a new type of label-free biochip for the prognosis of breast cancer.
Dr Philip Tinnefeld is Professor of Biophysical Chemistry at the Technische Universität Braunschweig where he runs a laboratory which specialises in the field of NanoBioScience. His group has pioneered the integration of DNA origami with optical single-molecule spectroscopy over the last few years.
Dr Rosalie Driessen is a post-doctoral researcher in the Laboratory of Molecular Genetics, a part of the Leiden Institute of Chemistry at Leiden University. She is studying the organization and dynamics of bacterial chromatin having received her doctorate from the University for her thesis, “The architects of crenarchaeal chromatin.”
Dr Rico Tabor leads the Soft Materials and Colloids Group in the School of Chemistry at Monash University. Research focuses on several areas. At a fundamental level, the group is interested in exploring the interactions that cause aggregation and assembly, in everything from nanoparticles, droplets and graphene to proteins and cells.
Professor Kawase from the Institute of Medicine and Dentistry at Niigata University uses QI™ mode and the NanoWizard® 3 AFM to determine the optimal stiffness or elasticity of cell scaffolding materials, and to demonstrate dynamic changes in cytoskeletal fiber formation in response to the mechanical property of scaffolds.
Dr Paul Squires and Dr Claire Hills from the School of Life Sciences (SLS) at the University of Warwick have international expertise in cell biology, signalling and endocrinology. They are using the JPK CellHesion® 200 system to study cellular adhesion in the field of diabetes.
Professor Yi Cao is a member of the Institute of Biophysics located in the Physics Department of Nanjing University. The main focus of his group is to study how force regulates the structures and conformational dynamics of biomacromolecules using AFM based single molecule force spectroscopy.
Dr Florian T. Ludwig is a member of the team of Professor Albrecht Schwab and Dr Christian Stock. These researchers are interested in how ion transport across the plasma membrane affects tumor cell motility and, thus, metastasis. Together with Mike Wälte from the group of the Head of the Institute, Professor Hans Oberleithner and Dr Hermann Schillers, Dr. Ludwig applies the CellHesion® 200 to measure cell-cell adhesion forces in tumor cells.
Dr Marit Sletmoen is an associate professor in the Department of Physics. She specialises in the study of biological polymers, biophysics and medical technology and applies the NanoTracker™ Optical Tweezers system in the study of biomacromolecules.
Dr Giovanni Longo under the supervision of Professor Giovanni Dietler at the "Laboratory of the Physics of Living Matter"(LPMV) of EPFL uses the NanoWizard® 3 AFM system to study bacterial resistance to antibiotics and the growth of osteoblasts with a focus on their interactions with differently prepared substrates and to different gravity conditions.
Professor Shinji Deguchi leads a research group at Nagoya Institute of Technology (NITech). The Laboratory for Mechanobiology & Bioengineering has a multi-disciplinary approach to the growing field of Mechano-Biology. This brings together the areas of cell biology, bioengineering and biophysics.
Professor Takeshi Fukuma runs a research group in the Division of Electronic Engineering and Computer Science at Kanazawa University in Japan. The Fukuma Laboratory focuses on molecular-scale analysis and measurements of biological phenomena using atomic force microscopy, AFM.
Dr Christian Marlière leads a research team to study the dynamics and properties (mechanical, electrical and electro-chemical) of living bacteria in purely controlled physiological conditions at nanometer scale: motility or adhesion processes of bacteria on solid substrates, biofilms formation, the influence of light on moving properties of cyanobacteria, effects of antibiotics on bacterial membranes and biofilm etc.
The research of the Yan Jie single molecule biophysics research group at NUS is based on highly sensitive single-molecule manipulation and imaging techniques. It also requires understanding mechanics of biopolymers.
The Nano-mechanics group develops and uses novel techniques to understand molecular processes and has recently developed new instrumentation in the fields of single molecule force spectroscopy and correlation spectroscopy.
The group of Dr Rikke Meyer works at the interface between microbiology and nanoscience in the quest to understand how bacteria form biofilms and how this may be prevented. AFM and optical microscopy are used to visualize bacterial cells and to study the interaction forces between cells and an abiotic substrate. AFM imaging and single-cell force spectroscopy are excellent tools to visualize detailed structures on the bacterial cell surface and to study how these contribute to cell adhesion to other substrates.
JPK Instruments reports on the use of AFM systems in the group of Dr Bart Hoogenboom of the London Centre for Nanotechnology. Dr Hoogenboom’s laboratory collaborates with JPK to look at ways to push instrumentation to new limits of resolution and imaging.
JPK Instruments reports on the use of their NanoWizard systems in the Microscopy and Imaging Facility (MIF) at the University of Calgary. Single cell force spectroscopy at the MIF has now attracted high profile research with three NanoWizard® AFM systems from JPK, one of which is equipped with the CellHesion® module.
Dr Higgins’s main interest and research has focused on the application of AFM to study the nanoscale interactions of biological systems, including living cells, model lipid membranes, single ligand-receptor interactions, individual protein unfolding, fundamental surface-force interactions, as well as being involved in AFM instrument development.
JPK Instruments reports on the multi-faceted research projects of Dr Jochen Guck who runs simultaneous research at the Universities of Dresden and Cambridge.
Dr Clemens Franz leads a group of researchers at the DFG-Center for Functional Nanostructures at Karlsruhe Institute of Technology where he works on expanding the use of AFM for cell biological applications.
JPK Instruments is pleased to report on the research work from the Chemical Physics group of Professor Robert Magerle of the Chemnitz University of Technology. The Chemical Physics Group is part of the Faculty of Natural Sciences. The main research topic is the study of the structure and properties of polymeric materials.
JPK Instruments reports on the work from the Pharmaceutical Sciences Department of the Medical School at the University of Nebraska. The Department have selected the JPK ForceRobot® 300 system to extend their studies applying atomic force microscopy, AFM, in the measurement of single molecule force spectroscopy.
The research of the Institute of Experimental Physics I is focused on soft condensed matter as bulk material, in interaction with surfaces and interfaces, and with single molecules. The systems under study cover a wealth like small tracer molecules, liquid crystals, polymers, polymer-networks, proteins, and even living biological cells. It is objective of the research of the Institute of Experimental Physics I to explore the physical basis of structure-property relationships in these systems.
JPK Instruments is pleased to report on the first work from the University of Nottingham who have chosen the ForceRobot® 300 system for their School of Pharmacy.
JPK Instruments reports on the work of the Stark research group at LMU-Munich in cooperation with the EURAC Bolzano where a NanoWizard® AFM system has been used to reveal the nanostructure and mechanics of mummified type I collagen from the 5300-year-old Tyrolean Iceman.
Featured user publications
CLAFEM: correlative light atomic force electron microscopy Janel S, Werkmeister E, Bongiovanni A, Lafont F, Barois N., Methods in Cell Biology 140 (2017), 165-185
Tissue stiffening coordinates morphogenesis by triggering collective cell migration in vivo. E. H. Barriga, K. Franze, G. T. Charras, R. Mayor. Nature 554 (2018), 523–527
Novel insights into cardiomyocytes provided by atomic force microscopy D. Borin, I. Pecorari, B. Pena, O. Sbaizero, Seminars in Cell & Development Biology 73 (2018) 4-12
Real-time TIRF observation of vinculin recruitment to stretched α-catenin by AFM K. Maki, S.-W. Han, Y. Hirano, S. Yonemura, T. Hakoshima and T. Adachi, Scientific Reports 8, Article number: 1575 (2018)