Specifications
- Innovative platform for cell adhesion/cell mechanics research or for other long range force experiments integrated into inverted research microscopes
- Compatible with inverted microscopes for combined experiments, e.g., Zeiss Axio Observer, Axiovert A1, Nikon Ti/TE 2000, Nikon Ti2, Olympus IX series and Leica DMi series
- Integrates with advanced optical imaging such as DIC, phase contrast, fluorescence, confocal microscopy, Superresolution.
- Compatible with upright macroscopes and stereo microscopes such as Zeiss AxioZoom V.16, Leica Z16 ApoA, Leica M205FA and Olympus MVX10
- Living cell studies in native environment with tempeature control, perfusion and gas flow (CO2) on coverslips with the JPK BioCell™ or 35mm Petri dishes with or without glass bottom from Wilco, BD, WPI with the JPK PetriDishHeater™
- All parts which came in contact with the sample are easy to clean
- Compatible with standard incubators (specify model)
- High-throughput determination of cellular interaction parameters through JPK software batch processing
- Single-molecule events, work of removal, maximum adhesion forces, number of unbinding events and viscoelastic parameters such as Young’s modulus in one system
- 100 μm additional z-range with capacitive sensors for sub-nm precision
- 100 μm focus tracking optional by PIFOC® system keeps the plane of interest in focus during force measurements (substrate or cell surface)
- Proven NanoWizard® AFM technology for high resolution imaging and force measurements
DOWNLOAD BROCHURELiterature
- Quantitating membrane bleb stiffness using AFM force spectroscopy and an optical sideview setup. Gonnermann et al., Integr. Biol. (2015)
- Atomic force microscope-based single cell force spectroscopy of breast cancer cell lines: An approach for evaluating cellular invasion. Omidvar et al., Journal of Biomechanics 47(13), 3373–3379 (2014)
- Auxetic nuclei in embryonic stem cells exiting pluripotency. Pagliara et al., Nature Materials 13, 638-644 (2014)
- Quantitative investigation of calcimimetic R568 on beta cell adhesion and mechanics using AFM single-cell force spectroscopy. Siamantouras et al., FEBS Letters 588(7), 1178-1183 (2014)
- Assay for characterizing the recovery of vertebrate cells for adhesion measurements by single-cell force spectroscopy. Schubert et al., FEBS 588(19), 3639-48 (2014)
- Dynamic coupling of ALCAM to the actin cortex strengthens cell adhesion to CD6. Te Riet et al., J. Cell Sci. 127(Pt 7), 1595–1606 (2014)
- Quantifying cellular adhesion to extracellular matrix components by single-cell force spectroscopy. Friedrichs et al., Nature Protocols 5(7), 1353-1361 (2010)