High-resolution imaging with extreme performance
The image shows atomic lattice of mica in buffer, taken in closed-loop on an inverted microscope.
- PeakForce Tapping® for easy imaging
- Fast Scanning option with up to 150 lines/sec
- NestedScanner Technology for high-speed imaging of surface structures up to 16.5µm with outstanding resolution and stability
- New tiling functionality for automated mapping of large sample areas
- V7 Software with revolutionary new workflow-based user interface
- DirectOverlay™ 2 software for perfect integration and data correlation with advanced fluorescence microscopy platforms
- Vortis™ 2 controller for high-speed signal processing and lowest noise levels
The image shows tailored DNA origami frames imaged in TAE 10mM MgCl2 buffer on mica. Sample courtesy of R. Willaert, VUB, Brussels (BE). Scan field: 125nm · Height range: 4.4nm · Scan speed: 150 lines/sec
Automated mapping of large sample areas with new tiling functionality
The HybridStage™ or Motorized Precision Stage transforms experiments by enabling direct access to a large sample area, with automated, motorized movement to selected positions, grids and mapping regions. Begin with the DirectOverlay 2 optical calibration, and then select a region for optical tiling up to millimeters in size.
Precise motor movements automatically bring the whole sample into view, making it easy to select regions and features for further investigation. A single click navigates from point to point or MultiScan experiments automate a sequence of measurements at selected points.
The images show living Vero cells in cell culture medium at 37°C in PetriDishHeater™ . Optical tiling with 5×6 phase contrast images covering a 630µm×450µm region. - Zoom into region scanned with AFM showing 100μm×100μm scan (height range 5μm) and inset 15μm×15μm (height range 2μm) scan topography images using PeakForce Tapping. The feedback correction signal images highlight the surface membrane features, particularly in the zoomed image. Microvilli dominate the center of the cell, with membrane ruffles at the cell boundary.
The NanoWizard 4 XP, with its unique tip-scanning technology and fast imaging capabilities, is ideal for taking advantage of the synergy between AFM and super-resolution microscopy. The NanoWizard 4 XP is compatible with a wide range of platforms, such as those from Zeiss (PALM/STORM, SIM), Leica (STED), PicoQuant(STED), Nikon (SIM, STORM) and Abberior (STED). The 980nm laser option for the AFM head allows the simultaneous use of optical microscopes and focus stabilization systems, critical for long term experiments, and avoids conflicts with fluorescence or spectroscopy measurements.
The images show a STED and AFM experiment of living A549 cells imaged at 37°C in medium. STED image of microtubules labelled with silicon rhodamine overlayed with AFM topography. AFM QI topography image at 240pN imaging force (height range 3.5μm). Corresponding QI Young‘s modulus image (z range 100kPa).
NanoWizard 4 XP setups on Zeiss LSM 880 confocal microscope with Airyscan , with Upright Fluorescence Microscope (UFM) Kit for tissues or other large samples such as organs , with BioMAT Workstation for high NA optics and Zeiss Axio Imager , with TopviewOptics and on on Olympus with PicoQuant MicroTime 200 STED.
Outstanding quantitative data from molecules, cells and tissues
QI™ Advanced, based on real force curves, offers both astounding speed and resolution for applications ranging from single molecules to living cells. The quantitative data allows precise and fast analysis of mechanical or biochemical interactions, e.g., localization of binding sites, directly overlaid with fluorescent labelling and topography with Molecular Recognition Imaging. Advanced batch processing options include multiple models for modulus fitting and can reveal surface topography at zero force with Contact Point Imaging (CPI).
Single cell force spectroscopy measurements using the CellHesion module with an increased z range of 100 µm, showing the detachment force curves of a single A549 cell from fibronectin (FN) and from bovine serum albumin (BSA) coated culture dishes. Note, that the detachment of the cell from fibronectin results in a very large pulling range of 77 µm.
- Stiffness mapping of non-cancerous human cervix tissue with a HybridStage. The inset in the fluorescently-labelled (Hoechst) slab was used for mapping of a 5×4 quadrant area of 1000μm×800μm with overlayed composite Young‘s modulus map shown in the middle panel. The representative topography channel from an individual 200μm×200μm channel is given in the right panel. Sample courtesy of Dr. T. Fuhs and Prof. J.A. Käs, University of Leipzig, Germany.
New workflow-based user interface redefines user-friendliness
The new V7 software interface guides users through the workflow to set up experiments intuitively and makes it simple, even for users with minimal AFM experience, to progress confidently to generating high-quality data. Each stage of the setup and operation works as an optimized desktop that brings all the vital information into focus with a single click.
- On-screen, context-sensitive help
- Status feedback for alignment and setup
- Efficient task-based experiment selection
- Fast access to favorite and recently used experiments
- One-click probe calibration
- Instant overview of the key data.
- User management for Multi-User environments like imaging facilities
- Fast Scanning option with up to 150 lines/sec for tracking dynamic processes
- Now with Bruker’s exclusive PeakForce Tapping as standard
- Atomic lattice resolution on inverted microscopes with a large scan field of 100 × 100 × 15 µm3
- New workflow-based user interface for ergonomics and ease of operation
- New tiling functionality for automated mapping of large sample areas together with the HybridStage
- Enhanced DirectOverlay 2 mode for most precise correlative microscopy
- New Vortis 2 controller with high-speed low-noise DACs and cutting-edge position sensor readout technology
- Highest flexibility and upgradeability with a broad range of modes and accessories