The BioCell™ allows for temperature-dependent study of single molecules in their native environment. It is therefore particularly suited for applications in cell biology and biomedical research. As an add-on for inverted optical microscopes the ForceRobot® 300 in combination with the BioCell™ offers the unique possibility to simultaneously study temperature-dependent processes like aggregation and binding with the SMFS and simultaneous advanced optical imaging. During force measurement all the optical microscope‘s operation modes, in particular contrast techniques, remain constantly available. Even high-resolution fluorescence confocal microscopy and single-molecule fluorescence techniques are possible since objective lenses with highest numerical aperture can be used.